Figure 4. Disruption of microtubule polymerization also rescued the reduction of actin comet formation induced by dynamin2 depletion.

HeLa cells were transfected with control or dynamin2 siRNA for 72 h and then infected with Listeria for 1 h. Cells were placed in medium containing colchicine (10 µM; lower panels) for 5 h. Cells were fixed and immunolabeled with anti-α-tubulin (A) or phalloidin (B). Bar, 20 µm. Arrows indicate actin comet tails. (C) The length of 10 actin comets was measured and is presented as the mean ± SD of 10 bacteria.