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. 2011 Dec 12;6(12):e28603. doi: 10.1371/journal.pone.0028603

Figure 5. Taxol treatment elongated actin comet in the presence of dynamin2 dominant negative mutant.

Figure 5

HeLa cells were transfected with dynamin2 WT-V5/His or dynamin2 K44A-V5/His for 24 h, and infected with Listeria for 1 h. Cells were placed in taxol-containing medium (50 µg/ml; lower panels) for 5 h. Cells were fixed and processed for immunofluorescence procedures. The expression of dynamin2 WT or K44A was confirmed by anti-V5 antibody (not shown). (B) The length of actin comets was measured and is presented as the mean ± SD of 10 bacteria. Bar, 20 µm.