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. 2011 Dec 12;6(12):e28723. doi: 10.1371/journal.pone.0028723

Figure 3. Conformational dependence of the interaction of non-visual arrestins with ERK2.

Figure 3

COS-7 cells were transfected with WT, 3A, or Δ7 mutant forms of Flag-tagged arrestin-2 (A) or arestin-3 (B), along with ERK2-HA, with or without HA-β2AR. Cells were serum starved overnight 24 hours post-transfection and treated for 10 min at 37°C with or without 10 µM β2AR agonist isoproterenol. Cells were lysed, and arrestins were immunoprecipitated with anti-Flag antibody, and co-immunoprecipitated ERK2 and β2AR were detected with anti-HA antibody. Bar graphs show the ratio of co-immunoprecipitated ERK2 to immunoprecipitated arrestin. The data from three independent experiments were statistically analyzed by ANOVA. The significance of the differences is indicated, as follows: * or &, p<0.05; ** or &&, p<0.01, as compared to corresponding within group basal level of ERK2 co-immunoprecipitation (black bars); a or $ or #, p<0.05 compared to WT control (black bar in WT group).