Figure 4. Quantification of antigen-specific T-cell responses by an acDC-amplified ELISpot assay.

GAD₆₅- and TTX-induced (A) IFN-γ and (B) IL-4 spot forming cells (SFC) in GAD-alum- (filled symbols, n = 15) and placebo-treated patients (open symbols, n = 12), expressed as SFC/10⁶ PBMC calculated from the mean of quadruplicates after background subtraction. Median and interquartile range is indicated. (C) Correlation between GAD₆₅-induced IFN-γ SFC, detected by acDC ELISpot and IFN-γ secretion, detected by Luminex, in GAD-alum treated patients. (D) Correlation between GAD₆₅-induced IL-4 SFC, detected by acDC ELISpot and IL-13 secretion, detected by Luminex, in GAD-alum-treated patients. Significant differences are indicated as p-values and correlation coefficient as r.