Table 2.
Primers used in this study
| Primers name | Nucleotide sequences |
|---|---|
| Construction of mutants | |
| fvtA mut-F | TTTTGAGCCAGTGGATCAGC |
| fvtA mut-R | CGTGTTTGTCAACATGGATCAG |
| fvtA mut-1 | CTAGAACTGGTAATCAGCGACATCCATGGGCTTCATGACCT |
| fvtA mut-2 | AGGTCATGAAGCCCATGGATGTCGCTGATTACCAGTTCTAG |
| fetA mut-F | TCAAGATGCGACTCTTCTGC |
| fetA mut-R | CTCAATGTGCTCCAAACCATC |
| fetA mut-1 | CCTAACCAATAACGACGTCCATTCAGCGGATTGAGTCTGG |
| fetA mut-2 | CCAGACTCAATCCGCTGAATGGACGTCGTTATTGGTTAGG |
| fetR mut-F | TTCAATGGCTTGCAGTGGTG |
| fetR mut-R | ACCAAGTGGAATCAGCCGTC |
| fetR mut Cla1-1 | GGTGCTTTCCATTCCTCAGGATCGATGGAAAGCTTTGATCGCACTG (underline, ClaI site) |
| fetR mut Cla1-2 | CAGTGCGATCAAAGCTTTCCATCGATCCTGAGGAATGGAAAGCACC (underline, ClaI site) |
| Complementation | |
| fvtA comp SphI F | GCATGCCGATAATGGTTGCAGCAACATC (underline, SphI site) |
| fvtA comp XbaI R | TCTAGACTAGAACTGGTAATCAGCGAC (underline, XbaI site) |
| fetA com SphI F | GCATGCCAAAATTATGAATAATCCACTCCTG (underline, SphI site) |
| fetA com EcoRI R | GAATTCTTAAAAATTAACGTCAATACCTAACC (underline, EcoRI site) |
| fetR com SphI F | GCATGCACAGATGGAATGGTCGATACCG (underline, SphI site) |
| fetR com XbaI R | TCTAGATTATGGTGCTTTCCATTCCTCAG (underline, XbaI site) |
| qRT-PCR | |
| fetA-QRT-F | TCACCTCATCACACGTGTTC |
| fetA-QRT-R | ATTTGGTCGAGTTTGACGCG |
| fetR-QRT-F | AAGGCAACCGTCTAACTTTGAC |
| fetR-QRT-R | TCCAGCCTTGTTGAGCAAAC |
| aroC-QRT-F | GTCGAATCCACCTTATGTGG |
| aroC-QRT-R | ACGGAATGTGGTCGTATTGG |