Table 3.

Primers used to genotype four SNPs in FGFR2^a

SNP	Primer	Primer sequence (5' to 3')	Product length	Annealing temperature	Further analysis
rs10736303	F	AGGGACAAATACTCCGCACA	405 bp	50°C	Sanger sequencing
	R	AGCCATCCAGCATGTTTCTC
rs2981578	F	TGACTCTTCAAAGTTTGTTTGTTTT	295 bp	50°C	Restriction enzyme AciI (New England Biolabs, Ipswich, MA, USA)
	R	GAGGAAAGGTTCCCCACACT
rs2981582	F	AGCTCAGCTTACCCCAGACA	215 bp	58°C	Restriction enzyme AciI (New England Biolabs, Ipswich, MA, USA)
	R	CGTGAGCCAAGCCTCTACTT
rs7895676	F	CAGGTGCGGTGGCTCATGTC	345 bp	67°C	Sanger sequencing
	R	GACTTCAATGGCGGGACTCC

^aFGFR2: fibroblast growth factor receptor 2 gene; F: forward primer; R: reverse primer; bp: base pair. For each SNP, the forward and reverse primer sequences, the length of the resulting PCR product and the annealing temperature used during PCR are shown. Also shown is the technique that was used on the resulting PCR fragment to genotype the SNPs.