Table 5.
Antibodies used during Western blot analysesa
| Antigen targeted | Antibody (catalog number, manufacturer) | Clonality | Dilution | Origin |
|---|---|---|---|---|
| Primary antibodies | ||||
| FGFR2 | Bek C-17 (sc-122, SZ) | Polyclonal | 1 in 500 | Rabbit |
| α-tubulin | Anti-α-tubulin (T6199, SA) | Monoclonal | 1 in 8,000 | Mouse |
| ERK1/2 | p44/42 MAPK antibody (9102, CS) | Polyclonal | 1 in 100 | Rabbit |
| Phosphorylated ERK1/2 | Phospho-p44/42 MAPK antibody (4377, CS) | Monoclonal | 1 in 200 | Rabbit |
| FRS2α | FRS2 H-91 (sc-8318, SZ) | Polyclonal | 1 in 50 | Rabbit |
| Phosphorylated FRS2α | Phospho-FRS2-α antibody (3864, CS) | Polyclonal | 1 in 50 | Rabbit |
| Secondary antibodies | ||||
| Mouse IgG | IRDye 680 anti-mouse IgG (926-32220, LC) | Polyclonal | 1 in 7,500 | Goat |
| Rabbit IgG | IRDye 800CW anti-rabbit IgG (926-32211, LC) | Polyclonal | 1 in 5,000 | Goat |
aFGFR2: fibroblast growth factor receptor 2 gene; ERK1/2: extracellular signal-regulated kinases 1 and 2; FRS2α: fibroblast growth factor receptor substrate 2α; IgG: immunoglobulin G; MAPK: mitogen-activated protein kinase. Manufacturer names are abbreviated as follows: SZ: Santa Cruz Biotechnology, Santa Cruz, CA, USA; SA: Sigma-Aldrich, Zwijndrecht, The Netherlands; CS: Cell Signaling Technology, Danvers, MA, USA; LC, LI-COR Biosciences, Cambridge, UK. For each antibody, the target, manufacturer, clonality, dilution used during Western blot analysis and origin are given.