Figure 6.

Effect of PPARγ overexpression on the growth characteristics of BCPAP cells. (a) 40 μg of nuclear extract protein from BCPAP cells transfected with either pQCXIP (empty) or pQCXIP-expressing PPARγ was separated on a 10% SDS-PAGE gel, transferred to PVD, probed with PPARγ antibodies (sc-7196), and reprobed with PARP as described in Figure 2(a). (b) Cell growth rate was assessed by counting viable BCPAP cells transfected with pQCXIP (empty) or pQCXIP-expressing PPARγ at 2-day intervals. Data is represented as fold cell number over day 0 (5 expts. ± SEM; *, P < 0.001 at day 6, P < 0.0001 at day 8 by 2-way ANOVA). (c) 50 μg of total cellular protein from BCPAP cells transfected with pQCXIP (empty) or pQCXIP-expressing PPARγ was separated on a 10% SDS-PAGE gel, transferred to PVD, and probed with antibodies against the corresponding cell cycle protein followed by the appropriate secondary antibody as described in Figure 4. β-actin was quantitated as a loading control.