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Millipore’s voltage-gated sodium channel IonWorks Quattro™ protocol. Cells are held at a voltage corresponding to ∼V1/2 inactivation, and subjected to 25 depolarization pulses to 0 mV for 20 ms. Pulses are applied every 100 ms. Data from Pulse 1 and Pulse 25 are reported to assess both tonic and use-dependent inhibition.
