Figure 3.

Quality-control data for Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.5, Nav1.6, and Nav1.7 assays collected over a 6 month period of time using IonWorks Quattro. Vehicle control was external buffer with 0.3% DMSO final concentration and reference inhibitors consisted of tetracaine (Nav1.1), lidocaine (Nav1.2, Nav1.3, Nav1.4, Nav1.5, Nav1.6), and lamotrigine (Nav1.7). Data shown were calculated from Pulse 1 and Pulse 25 (as in Figure 2) and displayed as percentage activation relative to the ratio of Post-Compound/Pre-Compound pulses. Where 0.3% DMSO had no effect between the Pre-Compound and the Post-Compound pulses, this was defined as 100% activation. (Top left panel) vehicle control data. (Top right) data obtained at P1 with the IC₅₀ concentration of inhibitor. (Bottom left) data obtained at P25 with the IC₅₀ concentration of inhibitor. (Bottom right) data from P25 using an IC₁₀₀ concentration of inhibitor_. All data points are from cells with a minimum of 250 pA of current, and the mean is shown as the large filled symbol in each panel. The IC₅₀ dose is chosen to estimate the block achieved at P25; since the compounds are state- and use-dependent blockers less than 50% block is frequently achieved at P1.