Figure 1.
Evaluation of spontaneous proliferation of PBMC from HTLV-1-infected individuals using flow cytometry. (a) Division index of cells. (b) Percentage of divided cells. Uninfected (n = 14) and HTLV-1-infected individuals (n = 45). The cutoff values to spontaneous proliferation were >0.06 for index division of cells and >5.8% for percentage of divided cells (three times the mean of index division of cells and percentage of divided cells for uninfected PBMC). (c) Correlation between the index division of cells and [3H]thymidine incorporation (peak cpm/1000). (d) Correlation between the percentage of divided cells and [3H]thymidine incorporation (peak cpm/1000). Differences were considered significant when P was <0.05 (Mann-Whitney U test). Spearman's correlation coefficients were used.