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Validation of lentiviral vector transduction and expression in D3H2LN cell lines. (a) Flow cytometer analysis of GFP-positive cells. Grey-filled area represents noninfected parental D3H2LN cells (Ctrl), and white area represents infected D3H2LN-AT2 (AT2) cells. (b) Biochemical validation of Flag-AT2 expression by Western blotting (anti-Flag-HRP) in total cell lysate (left panel) or in anti-Flag immunoprecipitate fraction (right panel).
