Table 1.
List of primers.
| Gene | Primer name | Size of product (bp) | Primer sequence (5′→3′) | Reference |
|---|---|---|---|---|
| (1) Detection of virulence gene | [26] | |||
| lukS-PV-lukF-PV | luk-PV-1 | ATCATTAGGTAAAATGTCTGGACATGATCCA | ||
| luk-PV-2 | 433 | GCATCAASTGTATTGGATAGCAAAAGC | ||
| tst | TST-1 | TTCACTATTTGTAAAAGTGTCAGACCCACT | ||
| TST-2 | 180 | TACTAATGAATTTTTTTATCGTAAGCCCTT | ||
| eta | mpETA-1 | ACTGTAGGAGCTAGTGCATTTGT | ||
| mpETA-3 | 190 | TGGATACTTTTGTCTATCTTTTTCATCAAC | ||
| etb | mpETB-1 | CAGATAAAGAGCTTTATACACACATTAC | ||
| mpETB-2 | 621 | AGTGAACTTATCTTTCTATTGAAAAACACTC | ||
| (2) SCCmec typing | [28] | |||
| (i) ccr gene complex type with mecA | ||||
| mecA | mA1 | TGCTATCCACCCTCAAACAGG | ||
| mA2 | 286 | AACGTTGTAACCACCCCAAGA | ||
| Type 1 | α1 | AACCTATATCATCAATCAGTACGT | ||
| βc | 695 | ATTGCCTTGATAATAGCCITCT | ||
| Type 2 | α2 | TAAAGGCATCAATGCACAAACACT | ||
| βc | 937 | ATTGCCTTGATAATAGCCITCT | ||
| Type 3 | α3 | AGCTCAAAAGCAAGCAATAGAAT | ||
| βc | 1,791 | ATTGCCTTGATAATAGCCITCT | ||
| Type 4 | α4.2 | GTATCAATGCACCAGAACTT | ||
| β4.2 | 1,287 | TTGCGACTCTCTTGGCGTTT | ||
| type 5 | γF | CGTCTATTACAAGATGTTAAGGATAAT | ||
| γR | 518 | CCTTTATAGACTGGATTATTCAAAATAT | ||
| (ii) mec gene complex class | ||||
| mecA | ml6 | CATAACTTCCCATTCTGCAGATG | ||
| mA7 | 1,963 | ATATACCAAACCCGACAACTACA | ||
| mecB | IS7 | ATGCTTAATGATAGCATCCGAATG | ||
| mA7 | 2,827 | ATATACCAAACCCGACAACTACA | ||
| mecC (C2) | mA7 | ATATACCAAACCCGACAACTACA | ||
| IS2(iS-2) | 804 | TGAGGTTATTCAGATATTTCGATGT | ||
| (iii) SCCmec type II subtyping | ||||
| IIa (II.1) | kdpB2 | TAAACTGTGTCACACGATCCAT | ||
| kdpB1 | 287 | GATTACTTCAGAACCAGGTCAT | ||
| IIb (II.2) | 2b3 | GCTCTAAAAGTTGGATATGCG | ||
| 2b4 | 1,518 | TGGATTGAATCGACTAGAATCG | ||
| IIE (II.3) | 4b3 | AACCAACAGTGGTTACAGCTT | ||
| 4b4 | 726 | CGGATTTTAGACTCATCACCAT | ||
| II.4 | II4-3 | GTACCGCTGAATATTGATAGTGAT | ||
| II4-1 | 2,003 | ACTCTAATCCTAATCACCGAAC | ||
| (iv) SCCmec type IV subtyping | ||||
| IVa (IV.1) | 4al | TTTGAATGCCCTCCATGAATAAAAT | ||
| 4a3 | 458 | AGAAAAGATAGAAGTTCGAAAGA | ||
| IVb (IV.2) | 4b3 | AACCAACAGTGGTTACAGCTT | ||
| 4b4 | 726 | CGGATTTTAGACTCATCACCAT | ||
| IVc (IV.3) | 4c5 | ATCCATTTCTCAGGAGTTAG | ||
| 4c4 | 259 | AGGAAATCGATGTCATTATAA | ||
| IVd (IV.4) | 4d3 | AATTCACCCGTACCTGAGAA | ||
| 4d4 | 1,242 | AGAATGTGGTTATAAGATAGCTA | ||
| (3) Multilocus sequence typing (MLST) | [27] | |||
| carbamate kinase (arcC) | arcC-Up | TTGATTCACCAGCGCGTATTGTC | ||
| arcC-Dn | 456 | AGGTATCTGCTTCAATCAGCG | ||
| Shikimate dehydrogenase (aroE) | aroE-Up | ATCGGAAATCCTATTTCACATTC | ||
| aroE-Dn | 456 | GGTGTTGTATTAATAACGATATC | ||
| Glycerol kinase (glpF) | glpF-Up | CTAGGAACTGCAATCTTAATCC | ||
| glpF-Dn | 465 | TGGTAAAATCGCATGTCCAATTC | ||
| Guanylate kinase (gmk) | gmk-Up | ATCGTTTTATCGGGACCATC | ||
| gmk-Dn | 429 | TCATTAACTACAACGTAATCGTA | ||
| Phosphate acetyltransferase (pta) | pta-Up | GTTAAAATCGTATTACCTGAAGG | ||
| pta-Dn | 474 | GACCCTTTTGTTGAAAAGCTTAA | ||
| Triosephosphate isomerase (tpi) | tpi-Up | TCGTTCATTCTGAACGTCGTGAA | ||
| tpi-Dn | 402 | TTTGCACCTTCTAACAATTGTAC | ||
| Acetyl coenzyme A acetyltransferase (yqiL) | yqiL-Up | CAGCATACAGGACACCTATTGGC | ||
| yqiL-Dn | 516 | CGTTGAGGAATCGATACTGGAAC |