Figure 4.

Compounds that increased mdx mBAM tetanic force included IGF-I (A); creatine (B); trichostatin A (C); prednisone, Solu-Medrol, and deflazacort (D); prednisolone, cortisol, and cholesterol (E); Juven and β-hydroxy-β-methylbutyrate (HDM) (F); and lisinopril (G). mdx mBAMs were cast on d 0, maintained in growth medium for 2 d, and switched to differentiation medium for 8–9 d when mBAM tetanic force had plateaued. mBAMs were then incubated for 3–4 d with the various compounds, and tetanic force was measured in MAD. Each point represents the mean ± se of 4–6 samples/group. Results are expressed as percentage change in tetanic force from initial tetanic force for each mBAM. Untreated control mdx BAMs were run in each experiment, and any drift from their test day 0 plateau value was used to correct the response of the test groups as described in Materials and Methods.