Figure 3.

Effect of MAA on externalization of PS in Ca Ski cells. Cells were treated with 60 μM of MAA for different time points. At 24 h of treatment, cells were treated with increasing concentration of MAA. At each time point, cells were collected and double stained with Annexin V-FITC and PI as described in Section 2. (a) Representative dual parametric dot plots of PI fluorescence (y-axis) versus Annexin V-FITC fluorescence (x-axis). (b) Bar charts showing the proportion of viable, early apoptotic, late apoptotic, and necrotic cells. (c) Bar charts show the percentage of Annexin V-positive cells (Annexin V+/PI− and Annexin V+/PI+). Values are mean ± S.E. of three experiments. Asterisks indicate a significant difference between untreated and treated cells (*P < 0.05).