Figure 2.
Expression and localization of endogenous Ca2+ signaling proteins in CFBE cells. A) Left panel: agarose gel showing that TRPC1 and TRPC4 were detected in CFBE cells by RT-PCR, but not other TRPC family members (TRPC2, 3, 5, and 6). Right panel: STIM1 and ORAI1 mRNAs were also expressed in both CFBE-WT and -ΔF508 cells. B) Endogenous Orai1, STIM1, TRPC4, and TRPC1 proteins are expressed at similar levels in CFBE-WT and -ΔF508 cells. C) Immunolocalization of Orai1, STIM1, CFTR, TRPC4, and TRPC1 in CFBE-WT and -ΔF508 cells. All these proteins were stained with Texas-Red, and nuclei were stained blue with DAPI. Scale bar = 20 μm. Note PM staining of Orai1, TRPC4, and WT-CFTR, and the perinuclear localization of TRPC1, STIM1, and ΔF508-CFTR.