Figure 9.

Orai1-mediated SOCE induces higher IL-8 secretion in CFBE-ΔF508 cells. A) Effects of CPA and PA on IL-8 secretion in CFBE-WT (black bars) and -ΔF508 cells (gray bars). CFBE cells were grown on filters to polarize and were left untreated or treated with 20 μM CPA or PA (10⁸ CFU/ml) for 4 h at 37°C. Samples were collected from the apical side of the filters, and IL-8 was measured by ELISA. Data are means ± se of 3 experiments. B) Inhibiting Orai1 dramatically reduces IL-8 secretion in CFBE-WT and -ΔF508 cells. Cells grown on filters were untreated or treated with 20 μM CPA with or without SOCE inhibitors (100 μM 2-APB or Ca²⁺-free solution). Data are means ± se of 5 experiments.