Table 4.
Numbers of cells injected and recovery of spermatogenesis in the recipient testes. The percentages of tubules are given for recovery of endogenous spermatogenesis (GFP-negative), donor colonies (GFP+), and donor colonies normalized to the average number of cells injected are shown. Values expressed as Mean ± SEM (N=10 testes for young, CY and cryptorchid; N=7 testes for adult).
Source of donor cells | Numbers of viable cells injected | Tubules showing endogenous recovery (%) | Tubules showing donor (GFP+) recovery (%) | Donor recovery normalized to average number of cells injected |
---|---|---|---|---|
Young | 0.19 ± 0.01 ×106 | 3.4 ± 0.8 | 13.8 ± 3.5 a | 15.0 ± 3.6 a |
Adult | 0.16 ± 0.02 ×106 | 4.6 ± 0.8 | 0.5 ± 0.2 b | 0.5 ± 0.3 b |
Cryptorchid | 0.15 ± 0.01 ×106 | 2.9 ± 0.6 | 6.7 ± 1.8 a | 7.5 ± 2.0 a |
Cyclophosphamide | 0.19 ± 0.01 ×106 | 4.7 ± 1.1 | 2.4 ± 0.8 b | 2.4 ± 0.8 b |
Different letters indicate statistically significant differences among all groups (p<0.05).