Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Magn Reson. 2011 Apr 9;211(1):37–44. doi: 10.1016/j.jmr.2011.03.020

Figure 1.

Figure 1

Timing diagrams for the pulse sequences. A. 15N-13C dipolar coupling / 13C chemical shift or 15N-13C dipolar coupling / 15N chemical shift separated-local-field (SLF) experiment. B. 15N/13C HETCOR experiment. C. 15N/13C HETCOR experiment with 1H-irradiation. SLF experiments provide the local fields (dipolar couplings) affecting on the observed spins in the t1 dimension, and the pulse scheme in the t1 dimension can be modified for different demands (1H-15N, 1H-13C, or 15N-13C dipolar couplings). Therefore, the chemical shifts acquired in the t2 dimension can be resolved by these two-dimensional experiments[31; 32]. CW refers to continuous wave irradiation and SPINAL-16[33] refers to the modulation used for heteronuclear decoupling. Pre-saturation is accomplished with thirty 90° pulses separated by 200 µs delay. The z-filter is 5 ms. Mix time for 1H/15N cross-polarization is 1ms, and for 15N/13C cross-polarization is 1ms or 3ms.