Table 1. Recombinant LigA proteins.
| Proteina | Amino acidcoordinatesb | MW (Da) | Primersc |
| LigA7′-13 | L631-P1224 | 63,422 | f-AACATATCTCATATGCTTACCGTTTCCAACACAAACGCCAAr-TTCCTCGAGTGGCTCCGTTTTAATAGAGGCTAAT |
| LigA7′-11 | L631-A1033 | 42,991 | f-AACATATCTCATATGCTTACCGTTTCCAACACAAACGCCAAr-GACGTCCTCGAGAGCAGAAGTGACATACAAGGTAGTAGA |
| LigA7′-9 | L631-A851 | 24,034 | f-AACATATCTCATATGCTTACCGTTTCCAACACAAACGCCAAr-AGTCTCGAGCGCTGCGGTAACGGATAATTTGGA |
| LigA10-13 | E852-P1224 | 40,602 | f-ACGCTTACGCATATGGAACTTACTGAGATTGTGCTAAATCCr-TTCCTCGAGTGGCTCCGTTTTAATAGAGGCTAAT |
| LigA10-12 | E852-E1124 | 30,085 | f-ACGCTTACGCATATGGAACTTACTGAGATTGTGCTAAATCCr-GCGTAGCTCGAGCTCGTCATTGACGAATATCCA |
| LigA11-13 | R943-P1224 | 31,233 | f-CATCAATGACATATGAGAATAGCTTCAATCGAAGTAACACCr-TTCCTCGAGTGGCTCCGTTTTAATAGAGGCTAAT |
| LigA11-12 | R943-E1124 | 20,716 | f-CATCAATGACATATGAGAATAGCTTCAATCGAAGTAACACCr-GCGTAGCTCGAGCTCGTCATTGACGAATATCCA |
| LigA12-13 | V1034-P1224 | 21,645 | f-ATACAGTCTCATATGGTCCTTATTGACATAGAAGTCAAGCCr-TTCCTCGAGTGGCTCCGTTTTAATAGAGGCTAAT |
a
LigA protein designations list the first and last domains included in the construct; 7′ indicates a half domain.
b
Coordinates refer to the first and last amino acids in the LigA protein of L. interrogans serovar Copenhageni strain Fiocruz L1-130.
c
Forward (f) and reverse (r) primer sequences, including an Nde I or Xho I site, respectively, are listed in the 5′ to 3′ direction.