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. Author manuscript; available in PMC: 2012 Dec 8.
Published in final edited form as: J Med Chem. 2011 Nov 9;54(23):8207–8213. doi: 10.1021/jm2012003

Figure 1.

Figure 1

Accumulation of adamantyl-tagged trioxolane derivatives within NLB of mature P. falciparum parasites is endoperoxide-dependent. Parasites were incubated with 500 nM of A) trioxolane 6; B) trioxolane 5, or; C) inactive trioxolane 7. Fluorescence from trioxolane 6, containing the cyclohexane dansyl fluorophore, distributes within the parasite cytoplasm uniformly (A) while the adamantyl-tagged trioxolane 5 signal is seen only within spherical NLB (B). In contrast to trioxolane 5, the inactive trioxolane derivative 7 distributes indiscriminately in the parasite, suggesting specific NLB localization requires endoperoxide activation. Images show merged fluorescence with phase contrast view (left) and fluorescence view only (right). Scale bars represent 5 μm.