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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Biomol Screen. 2011 Aug 5;17(2):237–244. doi: 10.1177/1087057111414903

Fig. 4.

Fig. 4

A scatter plot of the scintillation proximity assay (SPA) signals displaying the levels of methyltransferase activities of RPMT1 compared with the background signal level. Green line: the positive controls containing 0.9 μM 3H-SAM, 37 nM PRMT1, and 2.5 μM H4-BTN. Black line: the negative controls containing 0.9 μM 3H-SAM and 2.5 μM H4-BTN. Blue line: the inhibitor samples containing 0.9 μM 3H-SAM, 37 nM PRMT1, 2.5 μM H4-BTN, and 3 μM SAH. All reaction samples were incubated for 20 min, and following the reaction, 10 μL of 20 mg/mL SPA beads was added to each sample for scintillation counting.