Skip to main content
. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: J Control Release. 2011 Aug 7;157(1):132–140. doi: 10.1016/j.jconrel.2011.08.004

Fig. 6.

Fig. 6

(A). RT-PCR. Experiment was repeated three times with similar results.

(B) 1. Immunoblotting analysis of RRM1 in TC-1 and TC-1-GR cells. 2. Immunoblotting analysis of RRM1 in TC-1-GR cells transfected with RRM1-specific siRNA or control siRNA (Con). In A and B, β-Actin was used as an internal control.

(C). The IC50 values of didox in TC-1 and TC-1-GR cells. The cytotoxicity of didox was measured after 48 h of incubation. IC50 values were calculated from three different determinations.

(D). Cytotoxicity of gemcitabine in TC-1-GR cells transfected with RRM1-specific siRNA. Cytotoxicity of gemcitabine HCl at 1.6 μM and 35 μM was measured after 48 h of incubation. Con: cells transfected with negative control siRNA; siRNA: cells transfected with siRNA specific to RRM1. Data are presented as mean ± SD (n = 3) in C and D.