Figure 4.

Deceleration of [³H]NMS dissociation by MT7. The M₅-ELY and M₅-ELYE constructs were preincubated with [³H]NMS and dissociation initiated by addition of atropine with or without different concentrations of MT7. Samples were collected at different time points and [³H]NMS binding (B_t) determined. Data presented are from one experiment performed with triplicate samples. Two additional experiments with 10 and 100 nM MT7 gave similar results. Only the trendlines are shown for clarity. The control rate for M₅-ELY was 0.113 min⁻¹ and 0.003 min⁻¹ with 100 nM MT7. The control rate for M₅-ELYE was 0.147 min⁻¹ and 0.004 min⁻¹ with 100 nM MT7.