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An example of SRM detection of a selected peptide. A, the six 16O- and 18O-labeled transitions of peptide LFTGHPETLEK3+ in 1:5 molar ratio (16O:18O) in mouse plasma detected in accordance with expected ratio. Solid lines, 18O transitions; dashed lines, 16O transitions. B, relative intensities of transitions of the unlabeled peptide detected by LC-SRM-MS. C, relative intensities of transitions of the unlabeled peptide detected by infusion MS/MS of standard protein digest.
