Table I. A series of calibration mixtures made of mouse plasma and six standard proteins. The concentration values denote the concentrations in original plasma.
| Calibration mixture | Concentration of each standard protein in 18O-reference (% w/w) | Concentration of each unlabeled standard protein | Standard protein concentration ratio (unlabeled: 18O-reference) |
|---|---|---|---|
| M0 | 40 μg/ml (0.10%) | Blank | Background |
| M1 | 40 μg/ml (0.10%) | 400 ng/ml | 1:100 |
| M2 | 40 μg/ml (0.10%) | 800 ng/ml | 1:50 |
| M3 | 40 μg/ml (0.10%) | 1.6 μg/ml | 1:25 |
| M4 | 40 μg/ml (0.10%) | 4 μg/ml | 1:10 |
| M5 | 40 μg/ml (0.10%) | 8 μg/ml | 1:5 |
| M6 | 40 μg/ml (0.10%) | 40 μg/ml | 1:1 |
| M7 | 4 μg/ml (0.010%) | 20 μg/ml | 5:1 |
| M8 | 4 μg/ml (0.010%) | 40 μg/ml | 10:1 |
| M9 | 4 μg/ml (0.010%) | 100 μg/ml | 25:1 |
| M10 | 4 μg/ml (0.010%) | 200 μg/ml | 50:1 |
| M11 | 4 μg/ml (0.010%) | 400 μg/ml | 100:1 |