Table II. SRM quantification of 45 human plasma proteins with known relative abundance ratios relative to the labeled reference.

Protein	Peptide	Peptide replaced	Product ion	Slope ± CIa	y intercept ± CIa	R2	Accuracy (Error %)b	Mean % CV
Afamin	DADPDTFFAK		y7+	1.013 ± 0.018	0.036 ± 0.086	0.999	3.45	3.41
Albumin, serum	LVNEVTEFAK		y5+	0.968 ± 0.032	0.053 ± 0.151	0.997	2.17	2.42
α1-Acid glycoprotein 1	EQLGEFYEALDCLR	√	y8+	0.870 ± 0.028	0.137 ± 0.130	0.997	6.82	2.39
α1-Antichymotrypsin	EIGELYLPK		y2+	1.023 ± 0.007	0.025 ± 0.032	1.000	2.41	1.62
α1B-Glycoprotein	LETPDFQLFK		y8+	0.948 ± 0.028	0.141 ± 0.131	0.998	6.60	2.66
α2-Antiplasmin	LCQDLGPGAFR	√	y6+	0.984 ± 0.029	0.091 ± 0.135	0.998	4.04	3.27
α2-Macroglobulin	LLIYAVLPTGDVIGDSAK		y11+	0.985 ± 0.027	0.110 ± 0.129	0.998	8.36	2.04
Angiotensinogen	ALQDQLVLVAAK		y3+	0.982 ± 0.021	0.068 ± 0.097	0.999	4.66	3.52
Antithrombin-III	DDLYVSDAFHK		y6+	0.989 ± 0.054	0.040 ± 0.251	0.992	5.48	6.06
Apolipoprotein A-I	LLDNWDSVTSTFSK	√	y8+	0.976 ± 0.012	0.058 ± 0.055	1.000	4.64	1.06
Apolipoprotein A-II precursor	SPELQAEAK		y8+	0.947 ± 0.028	0.094 ± 0.131	0.998	6.16	3.43
Apolipoprotein A-IV	ALVQQMEQLR	√	y6+	1.003 ± 0.013	0.033 ± 0.059	1.000	2.54	2.70
Apolipoprotein B-100	LTISEQNIQR	√	y7+	0.993 ± 0.017	0.056 ± 0.078	0.999	4.03	4.76
Apolipoprotein C-I lipoprotein	EWFSETFQK	√	y7+	1.090 ± 0.037	0.060 ± 0.174	0.997	3.24	4.75
Apolipoprotein C-III	DALSSVQESQVAQQAR	√	y10+	1.025 ± 0.017	0.088 ± 0.081	0.999	5.36	2.97
Apolipoprotein E	LGPLVEQGR		y7+	1.027 ± 0.022	0.017 ± 0.102	0.999	3.02	2.73
β2-Glycoprotein I	ATVVYQGER		y6+	0.999 ± 0.006	0.032 ± 0.029	1.000	3.33	1.43
Ceruloplasmin	EVGPTNADPVCLAK	√	y6+	0.997 ± 0.026	0.037 ± 0.124	0.998	2.97	1.68
Clusterin	ELDESLQVAER		y3+	0.978 ± 0.022	0.050 ± 0.104	0.999	4.76	3.22
Coagulation factor XIIa HC	VVGGLVALR		y7+	0.969 ± 0.017	0.066 ± 0.081	0.999	6.17	1.93
Complement C3	AVLYNYR	√	y5+	0.987 ± 0.008	0.03 ± 0.039	1.000	2.69	1.32
Complement C4 β chain	VGDTLNLNLR		y3+	0.986 ± 0.009	0.022 ± 0.040	1.000	2.45	2.1
Complement C4 γ chain	VEYGFQVK	√	y6+	1.024 ± 0.015	0.001 ± 0.071	1.000	3.69	2.02
Complement component C9	VVEESELAR	√	y7+	1.014 ± 0.023	0.075 ± 0.108	0.999	5.49	2.61
Complement factor B	LEDSVTYHCSR	√	y6+	0.984 ± 0.048	0.002 ± 0.223	0.994	3.44	3.94
Complement factor H	EIMENYNIALR	√	y7+	0.897 ± 0.035	0.115 ± 0.163	0.996	3.24	3.62
Fibrinogen α chain	VQHIQLLQK	√	y7+	0.963 ± 0.018	0.057 ± 0.086	0.999	3.96	2.23
Fibrinogen β chain	EDGGGWWYNR	√	y4+	1.000 ± 0.013	0.047 ± 0.062	1.000	5.67	1.32
Fibrinogen γ chain	DNCCILDER	√	y4+	1.022 ± 0.018	0.014 ± 0.086	0.999	4.35	1.76
Gelsolin, isoform 1	TGAQELLR		y4+	0.984 ± 0.011	0.028 ± 0.043	1.000	0.88	3.01
Haptoglobin β chain	VGYVSGWGR		y6+	1.049 ± 0.006	−0.004 ± 0.030	1.000	1.05	0.78
Hemopexin	NFPSPVDAAFR		y7+	0.989 ± 0.013	0.030 ± 0.060	1.000	2.98	2.01
Heparin cofactor II	TLEAQLTPR		y6+	1.021 ± 0.022	0.031 ± 0.102	0.999	2.04	2.30
Inter-α-trypsin inhibitor HC	AAISGENAGLVR		y9+	1.016 ± 0.024	0.043 ± 0.111	0.999	3.40	2.07
Kininogen-1	YFIDFVAR	√	y6+	0.965 ± 0.010	0.065 ± 0.048	1.000	3.74	1.40
l-selectinc	AEIEYLEK		y5+	0.980 ± 0.141	0.731 ± 0.571	0.943	-	22.2
Plasma retinol-binding protein	YWGVASFLQK		y6+	1.021 ± 0.013	0.018 ± 0.059	1.000	2.97	4.17
Plasminogen	WELCDIPR	√	y6+	1.000 ± 0.012	0.038 ± 0.057	1.000	3.31	2.32
Prothrombin	YGFYTHVFR	√	y5+	1.064 ± 0.039	0.015 ± 0.185	0.996	3.03	4.49
Serum amyloid P-component	VGEYSLYIGR		y6+	0.972 ± 0.012	0.063 ± 0.058	1.000	1.81	2.16
Transferrin	EGYYGYTGAFR	√	y7+	1.012 ± 0.009	0.037 ± 0.043	1.000	2.86	0.94
Transthyretin	CPLMVK	√	y5+	0.994 ± 0.027	0.022 ± 0.110	0.998	1.90	3.32
Vitamin d-binding protein	THLPEVFLSK		y3+	0.951 ± 0.014	0.014 ± 0.064	0.999	4.00	2.66
Vitronectin	FEDGVLDPDYPR		y5+	0.961 ± 0.027	0.097 ± 0.127	0.998	4.28	3.95
Zinc-α2-glycoprotein	EIPAWVPFDPAAQITK		y10+	0.946 ± 0.017	0.064 ± 0.081	0.999	3.12	2.79
Averagec				0.991 ± 0.021	0.050 ± 0.096	0.999	3.79	2.67

^a CI, 95% confidence interval.

^b Accuracy was calculated based on the percentage of error relative to the known relative abundance ratios.

^c l-Selectin was excluded in calculation of the average values.