Table I. Genes and dedicated primers applied in the qRT-PCR.
Gene | Cluster | Accession N° | Stage: Protein list (N°) | Forward primer | Reverse primer | Amplicon (bp) |
---|---|---|---|---|---|---|
Sr-astacin | SR11111 | AAK55800 | iL3:Table II A (5) | TTGATACAGGAGTA | CCAACATATGA | |
AATGAAACTACAG | TCGACAACCA | 145 | ||||
Sr-ZK1073.1 | SR02886 | XP_001899587 | iL3: Table II A (2) | TTGTAGATTTGC | TCCCAAGCACTTT | |
CATTGCTCATCC | GAGTCATAATTC | 150 | ||||
Sr-PSC-1 | SR01641 | NP_971802 | pF: Table II B (7) | TGATGGTAAATTA | CATAATGTTGGATA | |
GATCGTGATGAGA | ATCTCTTCTGGTGA | 150 | ||||
Sr-calumenin | SR00564 | NP_001024806 | pF: Table II B (8) | TGATGGTAAATTA | CATAATGTTGGATA | |
GATCGTGATGAGA | ATCTCTTCTGGTGA | 149 | ||||
Sr-chitin binding protein | SR04455 | XP_001664881 | pF: Table II B (10) | ATGATACTAAGAA | GTATTGACCATCA | |
ACCTTTTACTCAAG | GGACATGAACTG | 152 | ||||
Sr-trypsin inhibitor-like protein | SR02054 | XP_001866937 | pF: Table II B (11) | CTTCCAACTGTC | CAGAAATACACTC | |
CAACAACTCAAA | ACATTTTGGTGGT | 150 | ||||
Sr-phosphoribosyl-transferase | SR02118 | XP_001895434 | pF: Table II B (22) | GGAACTGATTCA | TTGATGCTCCATT | |
ACTGGACATTTAC | GTCATTAACTGT | 151 | ||||
Sr-MFP2B | SR00863 | AAP94889 | flS: Table II C (2) | ATGCCAAATCTTAA | AGCTCTTCCATGA | |
ACCAGCTAAAGAAG | ATTGGTTTTCCAT | 150 | ||||
Sr-CBG22129; Y51F10.7 | SR02091 | XP_001667627 | flS: Table II C (4) | TCTCAAGGATTAG | TCCTTTATCATCAG | |
TACTTCCAAAAAC | TAATTTGAGCTTT | 150 | ||||
Sr-lysozyme family | SR00671 | NP_502193 | flS: Table II C (5) | TTACTGGATTCG | ACCAGCTTTCACA | |
ATGCCATTGGAA | GCATTTTTTATATT | 155 | ||||
Sr-galectin-2 | SR00627 | AAF63405 | All stages: Suppl. Table II (66) | CAAGCTGGAGAA | ATCACAACGATGA | |
TGGGGTAATGAGG | GCAAAAGTGCAG | 150 | ||||
Sr-galectin-1 | SS00840 | AAD39095 | All stages: Suppl. Table II (64) | GGAATGCCTGAAA | CTCTCTCTTCATT | |
AAAAAGGTAAACG | ACCCCATTCACC | 150 | ||||
Sr-GAPDH | SR00526 | NP_508534 | - | GTACCACTAAC | GCACCTCTTC | |
TGTTTAGCTCC | CATCTCTCC | 154 |