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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2011 May 19;1813(8):1453–1464. doi: 10.1016/j.bbamcr.2011.05.001

Fig. 2. AKT stabilizes both the nuclear and cytoplasmic fractions of FoxO3.

Fig. 2

HEK-293T cells transfected for 24 hours with control vector (lanes 15–18), HA-FoxO3 (lanes 7–14) or HA-FoxO3 3XA mutant (lanes 1–6) together with empty control vector (lanes 5, 6, 13, 14, 17, 18) GST control (lanes 3, 4, 9, 10), GST-AKT (lanes 1, 2, 7, 8, 15, 16) or GST-14-3-3ζ (11, 12) were fractionated to nuclear (N) and cytoplasmic (C) fractions. Immunoblot showing total FoxO3, pT32-FoxO3 and GST-fusion protein expression are presented. Alpha tubulin immunoblot served to verify the purity of the fractionations.