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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2011 May 19;1813(8):1453–1464. doi: 10.1016/j.bbamcr.2011.05.001

Fig. 7. 14-3-3ζ binding protects FoxO3 from dephosphorylation.

Fig. 7

HEK-293T cells were transfected for 24 hours with HA-FoxO3 together with GST (lanes 1–10) or GST-14-3-3ζ (lanes 11–20). Cells were treated with vehicle (lanes 1–5 and 11–15) or 10µM MG132 (proteasome inhibitor, lanes 6–10 and 16–20) for 2 hours, followed by treatment with 25µg/ml cycloheximide (protein translation inhibitor) for the indicated periods. Cells were lysed and equal protein amounts were analyzed for total and T32-phosphorylated FoxO3 levels by immunoblotting. Also presented are GST and tubulin immunoblots showing equal protein loading.