Figure 3. Experimental data vs. SL-E model simulation results for steryl-esters and total sterols.

After an external pulse bolus (Panels A–C). (A) Dynamics adapted from Taylor and Parks [42] after pulse-chase bolus of [¹⁴ C]-Acetate. (B) Simulation for total steryl-esters (X ₃₃+X ₃₄+X ₃₅+X ₄₀) and total sterols (X ₃₀+X ₃₁+X ₃₂+X ₃₆+X ₃₇+X ₃₉) after pulse bolus of labeled acetate. (C) Transported external label acetate (X ₁₂₅) and cytoplasmic acetate (X ₃₈). The initial label of external acetate was 125 µM. After 30 min pulse-chase with external [¹⁴ C]-acetate (Panels D–F). (D) Dynamics adapted from Taylor and Parks [42] after pulse-chase bolus of [¹⁴ C]-Acetate. (E) Model simulation for steryl-esters (X ₃₃+X ₃₄+X ₃₅+X ₄₀) and total sterols (X ₃₀+X ₃₁+X ₃₂+X ₃₆+X ₃₇+X ₃₉) after pulse-chase bolus of labeled acetate. (F) Transported external label acetate (X ₁₂₅) and cytoplasmic acetate (X ₃₈). The initial label of external acetate was 125 µM. The % Total Sterols was calculated as percent with respect to the total sterol amount for the S. cerevisiae wild type strain. In Fig. 3B and E, the “Normalized % Total Sterols” were normalized against the last experimental values for the steryl-esters from Figs. A and D.