Table 2. Steady-state values of PM ergosterol, total ergosterol, total ergosterol-esters, total sterols, and total steryl-esters in the SL-E model under different experimental conditions, after 60 minutes.
PM Ergosterol | Total Ergosterol | Total Erg. Esters | Total Sterols | Total Steryl Esters | |
X 36,X 37, X 39 | X 32, X 36, X 37, X 39 | X 35, X 40 | X 30, X 31, X 32, X 36, X 37, X 39 | X 33, X 34, X 35, X 40 | |
Wild Type (% total sterols) | 95.1 | 104.61 | 45.7 | 112.91 | 62.2 |
1% SPT | 0.96 | 0.97 | 1.05 | 0.98 | 1.05 |
1% SPT (120 min) | 0.89 | 0.92 | 1.11 | 0.94 | 1.12 |
1% IPC synthase | 0.94 | 0.95 | 1.02 | 0.95 | 1.01 |
12.5% Ceramide synthase | 1.00 | 1.00 | 1.01 | 1.00 | 1.01 |
Wild type condition is represented by the sum of the basal values; the other experimental conditions correspond to fold changes with respect to the wild type after decreasing the specific activity of serine palmitoyl transferase (X 157), IPC synthase (X 133), and ceramide synthase (X 134) to 1% or 12.5%.