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. 2011 Dec 14;6(12):e28531. doi: 10.1371/journal.pone.0028531

Figure 2. Time-course analysis mRNA for ER stress-related genes after Mtb H37Rv infection.

Figure 2

Raw264.7 cells were infected with Mtb H37Rv (MOI = 1) for 3 h, and then incubated for 0–6 h. (A) XBP-1 mRNA splicing was determined by RT-PCR using specific primers that was used to amplify products of unspliced and spliced mRNA. The results represent the ratio of XBP-1 splicing to XBP-1 unsplicing (XBP-1S/U ratio). (B, C) PCR amplification with the primer pair corresponding to the BiP and CHOP mRNA was performed at the indicated time points. The results were quantified by densitometry. For a positive control, cells were treated with 2.5 µg/mL tunicamycin (Tm) for 6 h. The statistical significance (*P<0.05, **P<0.01 and ***P<0.001) of observed differences between Mtb H37Rv infected and uninfected groups were verified by two-tailed t-test. Representative data from three independent experiments are shown.