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. 2011 Dec 14;6(12):e28611. doi: 10.1371/journal.pone.0028611

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EMCs expressing EGFP and cultured in DMEM or complete Medium 200 and HUVECs labeled with CellTracker™ Orange were plated on Matrigel individually or mixed at a 1∶2 ratio and imaged 5 and 20 hours later. The effect of 5 µM PEG-TNYL-RAW or an equal amount of PEG control on tube formation was analyzed. The histogram shows average tube lengths quantified from 2–3 micrographs at 5 hours for EMCs and 20 hours for HUVECs and HUVEC + EMC co-cultures and normalized to the average for the PEG control. Error bars represent the standard error from tube length measured from 3–4 wells. Tube length in the presence of PEG-TNYL-RAW was compared to that in the presence of PEG by one-way ANOVA and Bonferroni's post test. ***P<0.001. PEG-TNYL-RAW showed a trend towards decreasing tube formation in EMCs grown in Medium 200, which however did not reach significance (P = 0.11 by t-test). Scale bar = 250 µM.