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. 2011 Dec 14;6(12):e28611. doi: 10.1371/journal.pone.0028611

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HUVECs labeled with CellTracker™ Orange and EMCs expressing EGFP were plated on Matrigel in complete Medium 200 at a 2∶1 ratio and imaged 15 hours later. The effect of different concentrations of PEG-TNYL-RAW or TNYL-RAW on tube formation was analyzed. The histograms show the average tube lengths for the different peptide treatments normalized to the average for the PEG or DMSO controls. Error bars represent the standard error from 3–4 wells. Tube lengths for PEG-TNYL-RAW or TNYL-RAW were compared to the PEG or DMSO control by one-way ANOVA and Dunnett's post test. **P<0.001; ***P<0.001. TNYL-RAW at 20 µM showed a trend towards decreasing tube formation, which however did not reach significance (P = 0.40 by t-test). Scale bar = 250 µM.