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. 2011 Dec 14;6(12):e28611. doi: 10.1371/journal.pone.0028611

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Collagen embedded spheroids generated with HUVECs expressing mCherry, EMCs expressing EGFP or a 1∶1 mixture of the two cell types were treated with 5 µM PEG-TNYL-RAW or PEG for 2 days. The number of sprouts and the cumulative sprout length in the HUVE + EMC spheroids were normalized to the average for the PEG control. The histogram shows averages from 40–45 spheroids ± SE. The values obtained for spheroids treated with PEG-TNYL-RAW were compared to those with PEG control by one-way ANOVA. ***P<0.001. Scale bar = 250 µM.