Figure 5. The ΔkinA/ΔuncA/ΔuncB/alcA-myoV quadruple mutant exhibits a severe polarity defect when grown on the repressive YUU medium.

(A) The quadruple mutant failed to grow on a YUU plate. (B) In YUU liquid culture, the quadruple mutant failed to undergo polarized hyphal growth after an eight-hour incubation at 37°C. An arrow head points to a spore that had not undergone germination, thus indicating that mutant cells had undergone isotropic growth. (C) After an overnight incubation at 32°C, cells were highly abnormal in shape, and leakage of cell contents could be seen (arrows). An image of DAPI staining is included to demonstrate the presence of multiple nuclei. All panels shown are at the same magnification. Bar, 5 µm.