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. 2011 Dec 14;6(12):e28710. doi: 10.1371/journal.pone.0028710

Figure 5. Nephrin activation results in Lamellipodin recruitment.

Figure 5

A. Nephrin ligation results in generation of P(3,4)P2. Tapp1 PH domain is a specifically binds to P(3,4)P2. Human podocytes expression CD16-Nephrin and GFP-Tapp1 was clustered as described. There was recruitment of GFP-Tapp1 to CD16 Nephrin clusters suggesting generation of P(3,4)P2 proximal to the clusters at the membrane. B. Recruitment of Lamellipodin (LPD). Human podocytes expressing GFP-LPD were transfected with the indicated CD16-chimeric proteins. There was recruitment of GFP-LPD to CD16-Nephrin clusters but not to CD16-HA (control). C. Nephrin associates with Lamellipodin in a Ship2 dependent manner. Human podocytes expressing Flag-Nephrin were transfected with the indicated plasmids. Nephrin-LPD co-immunoprecipitation was evident only in presence of wild type Ship2. This was abrogated on transfecting a phosphatase dead mutant of Ship2 (D675A). Scale bar: 10 µm.