Figure 7. Ship2 knock down and lack of Filamin results in aberrant actin tails.

A. Human podocytes WT or Ship2^kd Hupo expressing the indicated plasmids were clustered as described and examined using confocal microscopy. M2 cells which lack Filamin were used to examine the actin tail formation in the absence of Filamin. B . Percentage of transfected cells with actin tails wider than 3 µm. Most cells with aggregated actin were easily identifiable. Image J software (National Institute of Health) was used to determine the width of the actin tails. Approximately 100 cells were analyzed in each condition. Data are representative of five different experiments. Scale bar: 10 µm.