Figure 1. End-joining efficiency is reduced when NHEJ factors are impaired.

(A) Scheme of the assay. Two I-SceI sites in reverse orientation are indicated by yellow arrow heads. The locations of the PCR primers used for the amplification of joined products are indicated by the red arrows. CMV: cytomegalovirus promoter/enhancer; IRES: internal ribosome entry site; pA: polyA signal. (B, C) siRNA- or inhibitor-mediated impairment of NHEJ proteins reduces NHEJ efficiency. (B) The results obtained 48 hr after the transfection of the I-SceI expression plasmid are shown. The proportion of eGFP-positive cells treated with siRNA or NU7026 (50 uM) is expressed as a ratio of values from siRNA-treated cells versus cells treated with non-targeting siRNA (siCTR) or DMSO. (C) The results of immunoblot analysis.