Table 1. Most common cis-acting promoter elements within the most differentially up-regulated genes in Arabidopsis germinated seeds at stage II treated with PEGa.
TF sites containing ABRE-motif | Consensus sequence b | P | S | P-value |
ABFs biding site motif | CACGTGGCACGTGGC | 36 | 41 | <10−10 |
AtMYC2 BS in RD22 | CACATGACATG | 102 | 142 | <10−4 |
ABRE-like biding site motif | BACGTGKMACGTGKM | 137 | 275 | <10−10 |
ACGTABREMOTIFA2OSEM | ACGTGKCCGTGKC | 128 | 217 | <10−10 |
CACGTGMOTIF | CACGTGACGTG | 104 | 302 | <10−10 |
GADOWNAT | ACGTGTCCGTGTC | 88 | 129 | <10−10 |
GBOXLERBCS | MCACGTGGCCACGTGGC | 32 | 36 | <10−10 |
ABRE binding site motif | YACGTGGCACGTGGC | 55 | 64 | <10−10 |
ABREATRD22 | RYACGTGGYRYACGTGGYR | 34 | 37 | <10−10 |
GBF1/2/3 BS in ADH1 | CCACGTGGCACGTGG | 14 | 28 | <10−5 |
Z-box promoter motif | ATACGTGTTACGTGT | 20 | 20 | <10−6 |
TF sites containing DRE-motif | ||||
DREB1A/CBF3 | RCCGACNTCCGACNT | 38 | 46 | <10−7 |
DRE core motif | RCCGACCCGAC | 91 | 122 | <10−10 |
Other TF sites | ||||
MYCATERD1 | CATGTGATGTG | 102 | 142 | <10−4 |
RY-repeat promoter motif | CATGCATGATGCATG | 19 | 38 | <10−4 |
Evening Element promoter motif | AAAATATCTAAATATCT | 32 | 38 | <10−4 |
Analysis performed with Athena [21].
Where B = T/G/C; K = T/G; M = A/C; Y = T/C; R = A/G and N = A/T/G/C.
The number of promoters containing at least one instance of the TF binding site and the total number of TF binding sites in the selected set of sequences are given in the ‘P’ and ‘S’ columns, respectively. The p-value for enrichment is a measure of how overrepresented a motif is in the selected set of genes versus the overall occurrence in the genome.