Figure 4. Expression of novel mRNA isoforms is both directly and indirectly regulated by androgens.

(A) UCSC Genome Browser showing chromosomal locations and annotated transcript details for each novel androgen-regulated alternative mRNA isoform. Custom tracks (black bars below “LNCaP AR ChIP-seq peaks”) for each gene show locations of AR binding site(s) within the LNCaP genome as determined by ChIP-Seq [4]. (B) Fluorescent quantification of capillary agarose gel electrophoretic analysis of RT-PCR products amplified from mRNA from LNCaP cells pretreated for 1 hour with vehicle (DMSO) or 1 g/ml cycloheximide (CHX) prior to addition of 10 nM R1881 for 24 hours. The relative normalised expression ratio is the contribution of the PCR product representing the alternative (androgen-regulated) transcript/constitutive transcript. Data from four independent replicates were used to obtain the means ± SE.