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. 2011 Dec 14;6(12):e29248. doi: 10.1371/journal.pone.0029248

Figure 3. Increased STAT1 phosphorylation in cells of AD-CMC patients.

Figure 3

(A) PBMC from two healthy controls and two AD-CMC patients were stimulated for two hours with culture medium, IFN-β (500 U/mL), IFN-γ (1 µg/mL), IL-23 (50 ng/mL) or IL-12 (10 ng/mL). Cells were lysed, and total STAT1, phosphorylated STAT1 and actin were assessed by Western blot. Figure is representative of two separate experiments. (B) Increased immunofluorescence of pSTAT1 in IFN-γ-stimulated CD4+ cells from AD-CMC patients. Figure is representative of three separate experiments. (C) PBMCs from three controls and two AD-CMC patients were stimulated with culture medium, IFN-β, or IFN-γ for two hours. The transcription of ICAM1 and MCP-1 was measured using RT-PCR. Bars represent means + SEM.