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. 2011 Dec 15;22(24):4706–4715. doi: 10.1091/mbc.E11-03-0251

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© 2011 Choo et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Role of CSN in preventing CAND1-mediated CRL disassembly. Cells were transfected with negative control or CSN5 siRNA, which was followed after 1 d by transfection of Cul1-V5. Cells were lysed 3 d after siRNA transfection, and cell lysates were subjected to V5 immunoprecipitation and Western blotting with the indicated antibodies.