FIGURE 8:

In vitro PtdInsP and PtdInsP₂ synthesis activation by c-Fos or c-Fos mutants. (A) PtdInsP and (B) PtdInsP₂ labeling was determined in vitro in the presence of c-Fos or its deletion mutants NA, NB, LZC, and ΔBD or its point-mutated versions K139N, R144N, and R146N resuspended in elution buffer to a final concentration of 1 ng/μg cell homogenate protein and ³²P-ATP. Control assays received an equal volume of elution buffer. Results are the mean ± SD of three experiments performed in duplicate. *p < 0.01 as determined by one-way analysis of variance with Dennett's post test. (C) Schematic representation of the c-Fos mutants used in A and B and/or for Figure 9. Note the relevance of BD for lipid synthesis activation.