FIGURE 9:

c-Fos mutants undergo FRET with CDS irrespective of containing or not containing c-Fos BD domain. (A) Cells cotransfected to express eYFP-CDS and the deletion mutants eCFP-NA (first row), eCFP-NB (second row), or eCFP-ΔBD (third row), the deletion mutant LZC (fourth row), or the point-mutated versions of c-Fos eCFP-R144N (fifth row) or eCFP-R146N (bottom row) were examined by confocal microscopy using filters for eCFP (left) or for eYFP (middle). FRET efficiencies images were obtained and pseudocolored using PFRET software (Elangovan et al., 2003; Chen et al., 2005) (right). (B) Mean FRET efficiencies ± SD for the donor/acceptor pairs shown in A. Results obtained after the examination of 50 cells in each case are from one representative experiment out of at least three performed. *p < 0.001 determined by one-way analysis of variance with Dennett's post test. Bar, 5 μm. FRET bar shown on the right corresponds to a blue-to-red increasing scale of FRET efficiency. Note that LZC, the deletion mutant lacking the N-terminus domain of c-Fos, fails to show association with CDS.