FIGURE 4:

Images from a time-lapse, alternating-frame FSM-DIC movie after generating a K fragment at anaphase onset (Movie S4). (A) FSM at metaphase. (B) DIC image of the released K fragment (red arrowhead) and polar basal body (green). (C–E) FSM at intervals after detachment of the K fragment to illustrate the lack of fluorescence at the plus end of the fragment's K fiber (red arrowheads) in comparison with fluorescence emerging from the kinetochores of controls moving to the same pole. Scale bar: 5 μm.