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. 2011 Dec 15;22(24):4842–4853. doi: 10.1091/mbc.E11-04-0348

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© 2011 Mattei et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Effect of PrP^C siRNA on PrP^C–tubulin association and apoptosis. (A) Left, cytofluorimetric analysis of fluorescence emission in CEM cells transfected with FITC-siRNA. The percentage of FITC-positive cells was considered indicative of the transfection efficiency. The number represents the percentage of FITC-positive cells (corresponding to transfected cells). Right, cytofluorimetric evaluation of PrP^C expression 72 h after siRNA transfection. The numbers represent the median fluorescence intensity and indicate the expression level of PrP^C. A representative experiment among three is shown. (B) Bar graphs showing quantitative cytofluorimetric analysis of apoptosis after cell staining with annexin V–FITC/propidium iodide. Values reported represent the percentage ± SD of positive cells obtained from three different experiments.