Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 15;22(24):4842–4853. doi: 10.1091/mbc.E11-04-0348

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Mattei et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 8: — Functional role of PrP^C in HeLa cells. (A) Crude mitochondria preparations from HeLa cells either untreated or treated with anti–CD95/Fas were obtained from HeLa cells by standard differential centrifugation and analyzed by Western blot. PrP^C localization was detected using an anti-PrP mAb (SAF 32). (B) Swelling induction in mitochondria. The swelling profile of mitochondria obtained from untreated HeLa cells as monitored by means of variations in TMRM fluorescence as a function of time. Results obtained in a representative experiment are shown. Note that 1) 300 μM calcium used as positive control induced a rapid loss of mitochondrial membrane potential, whereas calcium 10 μM was ineffective (left dot plot), 2) rec PrP was able to induce dose-dependent mitochondrial membrane hyperpolarization (middle dot plot), and 3) the supplement of 10 μM calcium after the PrP administration provoked a decrease of MMP in a significant percentage of cells (right dot plot). (C) Left, cytofluorimetric analysis of fluorescence emission in HeLa cells transfected with FITC-siRNA. The percentage of FITC-positive cells was considered indicative of the transfection efficiency. The number represents the percentage of FITC-positive cells (corresponding to transfected cells). Right, cytofluorimetric evaluation of PrP^C expression 72 h after siRNA transfection. The numbers represent the median fluorescence intensity and indicate the expression level of PrP^C. A representative experiment among three is shown. (D) Cytofluorimetric analysis of nonsilencing RNA (left) and siRNA (right) HeLa in the presence (bottom) or in the absence (top) of CD95/Fas treatment. Cell apoptosis was evaluated by staining with annexin V–FITC/propidium iodide. Values reported represent the percentage ± SD of positive cells obtained from three different experiments.