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. 2011 Dec 15;22(24):4854–4867. doi: 10.1091/mbc.E11-07-0592

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© 2011 Larimore et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Distinct mechanisms mediate the delivery of wild-type and dileucine mutant PI4KIIα into neurites. (A) Look-up table (LUT) of photobleached neurite tips of PC12 cells expressing EGFP-PI4Kα, EGFP-PI4KIIαL60-61A, or EGP-GAP43-ps. In vivo images were taken before (Pre), during photobleaching (0') and every 5 min thereafter for 30 min, after which an image was acquired every 15 min for an additional 45 min. (B, C) Time course of neurite tip fluorescence intensity (%) during FRAP, normalized to their fluorescence intensity before photobleaching. (B) EGFP-PI4KIIαL60-61A (n = 14 cells) recovers faster than EGFP-PI4Kα (n = 23 cells) following photobleaching, reaching a plateau within 10 min vs. 45 min, respectively. (C) No differences are observed in the time course of recovery between EGFP-PI4KIIαL60-61A and EGP-GAP43-ps (n = 8 cells). Scale bars, 20 μm.